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2- [tris (hydroxymethyl) methylamino] -1, ethanesulfonic acidum; Cas No.: 7365-44-8
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Synonyma: Chebi: (XXXIX) XXXVI, II - {[II-hydroxy-1,1-bis (hydroxymethyl), n-tris (hydroxymethyl), n-tris (hydroxymethyl (Hydroxymethylfonate; n-tris (II-ammonioethanesulfonate; n-tris (hydroxymethylfonfon (II-ammonioethanesulfonate
Chemical proprietas ex 2 [tris (hydroxymethyl) methylamino] -1 ethanesulfonic acidum
● species / color: album crystalline pulveris
● liquescens Point: ~ 223-225 ° C
● refractive index: 1.57
● PKA, 7.5 (XXV ad ℃)
● Psalm: 135.47000
● Density: 1.554 G / CM3
● Listp: -1.34880
● repono temp.: Copia ad Rt.
● Solubility.:h2o: I m ad XX ° C, patet, hyalinae
● aqua solubility.: Solutum
● Xlogp3: -5.8
● Hydrogenii Vinculum Donor Comes: IV
● Hydrogenium Bond acceptor comitem: VI
● Rotatable vinculum comitem: VI
● Missam exact: 229.06200837
● Gravis Atom comitem: XIV
● Complexity: CCXX
Notitia safty
● Picogram (s):
Xi
● Codes periculo:
● Dicta: 36/37/38
● Salutis statements: 22-24 / 25-36-26
Utilis
● canonica ridet:C (CS (o) (o) [O -]) [NH2 +] C (Co) (Co) CO
● utitur:A Structural Analog Tris quiddam. Biological quiddam. Tes est communiter usus builing agente.
Detailed introductio
2- [tris (hydroxymethyl) methylamino] -1, ethanesulfonic acidum, Etiam notum quod tes aut n-II-hydroxyethylpiperazine, n'-II-ethanesulfonic acidum, est zwitterionic buffering compositis communiter in biochemical et M. biology investigationis.
TesEst saepe usus est quiddam in officinarum experimenta debitum ad suam facultatem ponere firmum PH environment, praecipue in range of 6,5 ad 8.5. Etiam habet humilis ultraviolet lux effusio, quae facit idoneam usum in spectrophotometric Applications.This compositis est inerti et non interfere multis enzymaticum reactiones, permittens ad certum experimentis.
TesCommuniter in biologicum et biochemical assays, dapibus purificationis, electrophoresis, et cell culturae media.
Est dignum est attendendum quod tes est hygroscopic, id est facile absorbet humorem ex environment, ita est crucial ad reponere et tractamus recte ponere sua efficaciam.
Applicatio
2- [tris (hydroxymethyl) methylamino] -1 ethanesulfonic acidum (tes) est utile compositum praesertim notum est ad suum buffering proprietatibus. Quidam ex eius key usus sunt:
Buffering agente:Tes est communiter usus est quod buffering component in biochemical et M. biology experimenta. Adiuvat ponere firmum PH elit praecipue in range 6.5 ad 8,5
Biological et Biochemical Assays:Tes est usus in variis biologicum et biochemical assays in maintaining a specifica PH ad optimal praecessi est crucial. Is iuvat ponere PH de asay solutio et praebet stabilitatem ad reactionem conditionibus.
Purificacionis dapibus:Tes saepe usus est in dapibus purificationis processuum ex eius buffering facultatem et compatibility cum enzyme activities. Adiuvat ad stabilitatem et operatio servo durante purificatione vestigia.
Electrophoresis:Tes est usus est buffering component in gel electrophoresis techniques, praecipue in polyacrylamide gel electrophoresis (pagina). Hoc praebet a stabulo ph elit pro dapibus separationem et migrationem.
Cell Culture Media:Tes est includitur in cell culturae media ad ponere a constant PH ad optimal cellulam incrementum et proliferation. Culturi ad stabiliendum PH de cultura medium et providere idoneam elit pro cellulis.
Non est de ratione note quod ad specifica applicationem et concentration of tes ut variantur fretus experimentalem requisita. Proprie tractantem, repono, et operam ad details outlined in productum est technica documenta sunt maximus ut ad efficaciam et salutem.
