Productus
Hepes; Cas No.:7365-45-9
Synonyma: 1- [4 (II-hydroxyethyl) -1 -1 piperazinyl] ethane-II-sulfonicacid, 2- [4- (II-hydroxyethyl) piperazinyl] ethanylfonic acidum; 4- (II-hydroxyethyl), 1- (II, ethanEnsulfonic acidum); n- (II-hydroxyethyl) piperazine, n'-II-ethanesulfonic acidum; n- (II-hydroxyethyl) piperazine, n'-ethanesulfonic acidum; NSC (II-Hydroxyethyl), n- (II-ethanesulfonic acidum); II-II-hydroxyethylpiperazine, n-II-ethanesulfonic acidum, TVZ VII; erat XIII; Heepes (4- (II-hydroxyethyl), piperazine-I-ethanesulfonicacaca); I-piperazinezine, ethanesulfonicacac (II-piperazinezinezine, I-ethanesulfonicacac); I, hydroxyethanesulfonicacaacid); I-hydroxyethanesulfonfonicacaacid); I-piperazinezine, I-ethanesulfonicacac); I-piperazinezine) -:
Eget proprietas hepes
● species / color: album crystalline pulveris
● Vapor pressura, 0PA ad XXV ℃
● Melting Point: 234-238 ° C
● Refractive Index: N20 / D 1.339
● ferveret: CDVIII ℃ [In CI CCCXXV PA]
● PKA, 7.5 (XXV ad ℃)
● Psa:89.46000
● Density: 1.325 G / CM3
● Listp: -0.55930
● PRAECLUSIO TEMP.:2-8M
● Solubility.:h2o: I m ad XX ° C, patet, hyalinae
● aqua Solubility.:Solble
● Xlogp3: -4.1
● hydrogenii vinculum donator comitem: II
● Hydrogenium Bond Body comitem: V
● Rotatable vinculum comitem: IV
● Missa: 238.09872823
● Gravis Atom comitem: XV
● Complexity: CCLIV
Notitia safty
● Picogram (s):
Xi
● Codes periculo:
● Dicta: 36/37/38
● Salutis statements: XXIV / 25-22-36-26
Utilis
Canonica ridet:C1CN (CC [+ NH] 1cs (o) (o) [O -]) Cco
Description:Hepes sunt descriptus est unum de optimis omnibus, ad buffers available for biologicum investigationis. Biological PH, Molecule est zwitterionic, et efficax est quiddam ad PH 6,8 ad 8.2 (PKA 7.55). Est typically in cell culturae ad concentration inter 5mm ad XXX mm. Hepes sunt usus est in varietate applications, inter TEXTUS culturae. Est communiter ad quiddam cellulam culturae media in aere. Hepes invenit suum usus in vitro experimenta in mg.
Usus:Non-toxicus ad cellulam. Est usus ut hydrogenii Ion quiddam, quod potest control constant PH range enim diu term. Concentration 10-50mmol est / l. Plerumque, 20mMol / LhePes in CIBUS solutio can adepto quiddam habebat. Biological quiddam. Hepes est commune quiddam pro biologicis scientia, praecipue in cell culturae ponere physiologica PH. Actus est buffering component, quae est in praeparatione buffers. Describitur ut unum de optimis omnibus, ad buffers available for use in biologicum investigationis ,hepes est usus ut pars: Hank scriptor libratum sal solution, dulbucca scriptor modified Aquila conductatione et non-Basolatal latus in localized ad examents in Basolatal Selectived in localized in examine catione selectivity.
Detailed introductio
HepesStat pro 2- (4 (II-hydroxyethyl) piperazin-I-yl) ethanesulfonic acidum. Est a buffering agente vulgo in biologicum et biochemical investigationis. Hepes adjuvat ponere a firmum PH in cell culturae media, ut possit utraque accipere et donate protons, ita regulating pH mutationes. Eius chemical structuram et proprietatibus facere altus efficax in maintaining assidue et optimal PH pro multis biologicum et enzymaticum profectae. Hepes est communiter in cell culturae, dapibus purificationis et M. Biology artes.
Applicatio
Hepes habet plures applications in variis investigationis agris. Quidam ex communi applications hepes sunt:
Cell Culture: Hepes est communiter usus est pars in cell culturae media ad ponere a firmum PH environment. Hoc adjuvat moderari pH mutationes per metabolicae actiones et cumulum vastum products in cell culturas.
Enzyme Assays:Hepes est saepe assueti ut a buffering agente in enzyme assays ponere constant PH, quae est crucial pro propriis muneris de enzymes. Hoc adjuvat accurate et reproducible results.
Electrophoresis:Hepes adhibetur ut a buffering agente in electrophoresis techniques sicut polyacrylamide gel electrophoresis (page) et Agarose gel electrophoresis. Is iuvat ponere desideravit PH range ad optimal separationem et analysis de Macromolecule ut proteins et nucleic acida.
Purificacionis dapibus:Hepes est aliquando includitur in dapibus purificationem buffers ad ponere desideravit PH per diversas gradus ad purificationem processus. Is iuvat ponere stabilitatem et operatio de proteins in purificationem vestigia.
M. Biology Techniques:Hepes potest esse in variis M. Biology techniques, ut PCR (Polymerase catena reactionem) et DNA sequencing, ut ponere a firmum PH. Is iuvat optimize ad perficientur de enzymes et ensures accurate et reliable results.
Est momenti ad note quod specifica intentionem hepes et ad applicationem potest variantur fretus in experimentalem condiciones et requisita.
