Productus
Hepps; Cas No.: 16052-06-5
Synonyma: Hepps; 16052-06-5; EPPS; 4052-06-5; 4- (II-hydroxyethyl) -1 -1 piperazinepropanesulfonic acidum; 3- (4- (II-hydroxyethyl) piperazin-I-yl), I-sulfonic acidum; 3 [4- (II-hydroxyethyl) -1 piperazinyl] propanesulfonic acidum, I-piperazinepropanesulfonic acidum, 4- (II-hydroxyethyl), piperazin-I-yl], I-sulfonic-I-sulfonic- ACID;N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid);4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid;C9H20N2O4S;4-(2-Hydroxyethyl)-1-piperazinepropanesulphonic acid;EINECS 240-198-8;BRN 3957385;8JN5PQW99C;4-(2-Hydroxyethyl)-1-piperazinepropane sulfonic acid;MFCD00006160;3-[4-(2-Hydroxyethyl)-1-piperazinyl]-1-propanesulfonic Acidum; Ep1; n-II-hydroxyethylpiperazine, n'-III-propanesulfonic acidum, Unii-8jn5pqw99c; Schembl98490, DTXSID3066003, Chebi: (XLII) CCXCVIII; EPPS,> = 99.5% (Unum); Hy-D0873; C9-H20-N2-O4-S, BBL023080, MFCD12546632; STL356002, AKOS005267107, EPPS, Bioultra005205, Vetec (TM), Gale 99%;AC-24630;AS-69273;EPPS, BioXtra, >=99.5% (titration);LS-113260;CS-0015183;FT-0616538;H0576;F20313;M01848;A810147;N-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acidum; N- (hydroxyethyl) piperazine, n'-propany sulfonic acidum, n-II-hydroxyethylpiperazine-n-III-propanesulfonic Acidum; Q1563525; (CVII) CMLXXVI, 3 [4- (II-hydroxyethyl), -1 -1-III-hydroxyethyl) -1-piperazine-III-Hydroxyethyl) -1 -1-III-Sulfonic acidum; N- (II-hydroxyethyl) piperazine, n - (propanesulphonic acidum), 3- (4- (II-hydroxyethyl (hydroxyyl), Piperazinyl (II-Hydroxyeth), Piperazinyl] -1 -1) -1: EPPS, Bioparformance Certified, cell culturae probata,> = 99,5% (titrationis); 3- [4 (II-hydroxyethyl) piperazin-I-yl] propylsulfonoxoxion
Eget proprietas hepps
● species / color: album crystalline pulveris
● liquescens loco: 237-239 ° C (Lit.)
● Refractive Index: 1.537
● PKA: 8,0 (XXV ad ℃)
● Psa:89.46000
● Density: 1.285 G / CM3
● Listp: -0.16920
● repono temp.store ad Rt.
● Solubility.:h2o: I m ad XX ° C, patet, hyalinae
● aqua solubility.:soluble in aqua. 25.2 G / L (XX ° F)
● xlogp3: -3.6
● hydrogenii vinculum donator comitem: II
● Hydrogenium Bond acceptor comitem: VI
● Rotatable vinculum comitem: VI
● Missam exact: 252.11437830
● Gravis Atom comitem: XVI
● Complexity: CCLXXX
Notitia safty
● Picogram (s):
Xi
● Codes periculo:
● statements: XXXVI / XXXVII / 38-22
● Salutis statements: XXIV / 25-36-26
Utilis
Euge classes:NITROGENIUM COMPLENDIS -> Piperazines
Canonica ridet:C1cn (ccn1cccs (o =) (o) o) Cco
Usus:EPPS est communiter usus bono? Suffer. Saepe usus est ut SEPARATOR in ultathin Isoelectric focusing gels et enhances resolutio phosphoglomuperase. Est usus est ut a buffering agente in biology et biochemistry.
Detailed introductio
EPPS stat pro N- (II-hydroxyethyl) piperazine, n - (III-propanesulfonic acidum). Est zwitterionic buffering agente quod est communiter in biologicum et biochemical investigationis nonnullus key puncta de EPPs:
Buffering Properties:EPPs est efficax buffering agente quod adjuvat ponere constant PH in experimentalem setups. Is est a Pka valorem 8.09, quod facit idoneam ad buffering in phalicis de 7.0-9.0.
Stabilitatem:EPPS exhibet bona stabilitas super amplis temperaturis et minus affectus temperatus mutationes comparari aliis buffering agentibus sicut tris et phosphate buffers. Hoc stabilitatem facit idoneam usum in biochemical et biologicum experimentorum, qui requirit imperium PH condiciones.
Biological Applications:EPPs communiter in variis biologicum studia, ut dapibus purificationem, enzyme assays, et DNA / RNA. Et buffering capacitatem et stabilitatem ad physiologica PH faciunt illud valuable in his applications.
Cell Culture:EPPS potest etiam esse ut a buffering agente in cell culturae media. Is iuvat ponere a firmum ph ORGMITRIGATIO ad incrementum et sustentacionem quaedam cellula types.
Iones et compatibility:EPPs est zwitterionic compositis, id est continet utrumque positivum et negativum criminibus. Hoc pluma concedit ad efficaciter quiddam tam acidic et basic solutions. Est compatible cum variis biologicum moleculis et notum habere minimal intercessiones cum enzymatic profectae.
Cum usura lps, est momenti sequi commendatur concentration et PH guidelines provisum est a manufacturer. Praeterea, semper exercere cautionem sequi propriis salutem protocols cum tractantem quis eget aut buffering agente.
Applicatio
Hepps (N- (II-hydroxyethyl) piperazine, n'-III-propanesulfonic acidum) est zwitterionic quiddam quod est communiter in biologica et biochemical investigationis. Quidam ex eius applications includit:
Cell Culture: Hepps adhibetur ut a buffering agente in cell culturae media ad ponere constant PH et providere a idoneam environment in cellulam incrementum et proliferation. Is adjuvat stabiliendum PH intra physiological range et ne quis adversa effectus in cellular processibus.
Enzyme Assays:Hepps est saepe usus est quiddam in enzyme assays ad optimize ad PH condiciones requiritur ad maximum enzyme operatio. Et buffering capacitatem iuvat ponere firmum PH, cursus accurate et reliable enzyme mensuras.
Purificacionis dapibus:Hepps potest adhiberi ut quiddam in dapibus purificationis processus. Adjuvat ponere stabilitatem et operatio in proteins in purificationem vestigia, ut columna chromatography et dapibus purificationem per affinitatem tags.
Electrophoresis:Hepps est aliquando usus est quiddam in Gel electrophoresis ad separationem et analysis de proteins et nucleic acida. Eius PH buffering capacitatem adjuvat ponere firmum PH per electrophoretic currere, adiutricem in accurate separationem moleculis.
M. Biology Techniques:Hepps potest esse ut quiddam in variis moceular biology artes, comprehendo PCR (Polymerase catena reactionem), DNA sequencing, et enzymatic reactiones. Et buffering capability ensures optimal pH condiciones pro felix et efficient motus.
Studies involving membrana proteins:Hepps est bene apta studeo membrana proteins debitum ad humilitatem membrana penetrantia proprietatibus. Saepe usus est ut quiddam in experimentorum involving ad munus characterization seu recessio de membrana proteins.
Est momenti ad note quod meliorem concentration et PH de hepps potest variantur secundum specifica applicationem vel experimentalem condiciones. Unde non est visibilis ad consule litterae vel elit commendatione ad convenientem usum et concentration hepps in experimenta.
