Productus
Pipes; Cas No.: 5625-37-6
Synonyma:1,4, piperazinedhane sulfonic acidum;
1,4-piperazinedhanenhanenhanenhanetulphonichic acidum;
1,4, piperazineethanesulfonate, piperazine, n, n'-bis (II-ethanesulfonic acidum);
piperazine, n, n'-bis (II, ethanesulfonic acidum), sodium salis; Pipes
Chemical res Pipes
● species / color: alba / patet crystallina pulveris
● Melting Point:> CCC ° C (Lit.)
● refractive index: 1.6300 (estimate)
● PKA: 6.8 (XXV ℃)
● Psalm: 131.98000
● Density: 1.505 G / CM3
● Listp: 0.41700
● repono temp.store ad Rt.
● Solubility.Iv.1 m Naoh: 0,25 G / ML, patet, hyalinae
● aqua solubility.:soluble in aqua.
● Xlogp3: -6.7
● hydrogenii vinculum donator comitem: II
● Hydrogenii Bond acceptor comitem: VIII
● Rotatable vinculum comitem: VI
● Missa: 302.06062865
● Gravis Atom comitem: XVIII
● Complexity: CCCXCVII
Puritas / Quality
XCIX% * data a rudis Suppliers
1,4, piperazinebis (Ethanesulfonicacid) XCIX% * data ex Reagent Suppliers
Notitia safty
● Picogram (s):
Xi
● Codes periculo:
● Dicta: 36/37/38
● Salutis statements: 22-24 / 25-37 / 39-26
Utilis
Euge classes:Alia uses -> biologicum buffers
Canonica ridet:C1cn (ccn1ccs (o) (o) o) ccs (o) (o) o) Domine
Usus:Piperazine, N, N'-Bis- (II-Ethanesulphonic Acidum) Ultra -pure> 99.5%) est momenti eget intermedia in omnibus eget agros. 1,4, piperazinediethanenelfonic acidum, piperazine, n, n'-bis- (II-ethanesulfonic acidum) (tibia) est etiam unus de ZOGERS Buffers et maxime oportet quiddam in Pipes est 6.8 ad XX gradus Celsius. A buffering agente cum PKA circa physiologica Ph.pipes [piperazine, n, n'-bis (II-ethanesulfonic acidum)] est saepe usus est in buffering agente in Biochemistry. Est et ethanesulfonic acidum quiddam developed per bonum et al. In 1960s. Pipes habet PKA circa physiologica PH qui facit utile in cell culturae opus. Hoc est Documenta ad minimize lipid damnum cum buffering glutaraldehyde histology in planta et animal TITIS.Additional Forms Available: Pipes, SESQUISODIUM salis; Pipes dipotassium sal; Fistulae sphærarum; Pipes, sodium sal. Buffers potest parari per addendo solutio basi ad tibiae liberum acidum, titrating ad opportunitate PH, aut per miscentes aequimolaris solutions de Monosodium sal et cor. Pipes, adhibetur buffering agente in Biochemistry. Pipes habet PKA (6.76 at XXV ° C) apud physiologica PH qui facit utile in cell culturae opus. Protocolla sunt relatum in usum tibiae de separatione gyoxytis RNA in Agarose gels, nuclease S1 mapping of RNA et in Ribonuclease praesidio Protocols. Pipes dictum est ut quiddam in Glutaraldehyde fixation of TEXTUS exempla., Pipes dictum est usus in dapibus crystallization., Usus tibiarum in reconstrutionem ex animo in immunoadsorbent gels est descriptus. Pipes est suadetur pro usu in buffers ad in vitro studio Caspases III, VI, VII et VIII. A editis studium demonstrandum utilitatem in tali applications ut dapibus ion complexis in tali applications sicut dapibus ion complexione in tali applications sicut dapibus ion complexis quiddam in tali applications sicut dapibus ion complexis in tali applications sicut dapibus. Pipes est usus in cell culturae pro tali applications ut ipsum de thermMostable mutant membrana interdum in Esaichia coli.
Detailed introductio
Pipes libera acid, etiam notum quod 1,4-piperazinediethanenylfonic acidum, est bonum scriptor quiddam insignes habens PKA valorem similis ordinaria physiologica PH. Ut tale, fistularum liberum acid est saepe usus est in buffer in biochemical investigationis sit zwitterionic, piperazinic quiddam, quod est utile pro a range of 6.1 - 7.5. Pipes habebat facultatem ad formare significant complexu cum maxime metallum ions et commendatur ad usum sicut non-coordinating quiddam in solutions cum metallum ions. Pipes habet varietate applications et communiter in cell culturae media, in dapibus crystallization, ut currit Buffer in Gel electrophoresis, et quod Eluent in Isoelectric focusing et chromatographs. Hoc buffer capax formandi radicalis et ideo non idoneam redox profectae. Est idoneam usum in Bicinchoninic Acidum (BCA) Asay. Solubility Pipes crescit cum libera acid convertitur ad sodium salt.buffers fiunt per addendo basi solution ad tibias liberum acidum et titrato ad desideratum PH
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Cum suam PKA ad XXXVII ° C prope physiologica PH, Pipes habet applications in cell culturae opus.
Detailed introductio
Pipes etiam notum ut piperazine, n, n'-bis (II, ethanesulfonic acidum), est quiddam communiter in biologicum et biochemical investigationis. Est zwitterionic compositum quod adjuvat ponere firmum PH in aqueum solutions.
Una ex prima applications de tibiarum in agro M. Biology et Biochemistry, ubi est usus ut quiddam ad praeparationem et repono de biological exempla. Pipes habet PKA valorem de circiter 7.46, quod facit ideale pro maintaining a phalicus de 6.7 ad 7,9 at physiologica temperaturis (circa XXXVII gradus Celsius).
Pipes est praecipue utilis ad maintaining a firmum PH per processuum ut dapibus purificationis, enzyme assays, et gel electrophoresis. Is adjuvat ad minimize PH mutationes, quae non potuit in potentia afficiunt structuram et munus proteins et enzymes.
Alius application of Pipes est in cell culturae investigationis. Est communiter ut quiddam in cell culturae media. Pipes adjuvat ponere constant PH in incrementum medium, quod est crucial pro incrementum et viability cellulis. Etiam praebet stabile environment pro studeo cellular processibus et faciendi experimenta.
Pipes etiam in variis analytica techniques, ut liquid chromatography et capillaribus electrophoresis. Eius buffering capacitatem et PH stabilitas facere idoneam ad usum in his artes, ubi praecisa PH imperium est essentiale pro accurate results.
Praeterea, tibiae invenitur applications in medicamento formula et traditio systems. Potest adhiberi ut quiddam in pharmaceutical formulations ad optimize medicamento stabilitatem et augendae medicamento effusio.
Altiore, tibiae est a versatile buffering agente cum amplis applications in biologicum et biochemical investigationis, cell culturae, analytica artes, et pharmaceutical formulations. Et facultatem ad ponere a firmum PH facit valuable tool in variis scientific agri.
